comparison of the proteome profiling of iranian isolates of leish¬mania tropica, l. major and l. infantum by two- dimen¬sional electrophoresis (2-de) and mass-spectrometry
نویسندگان
چکیده
background: the mechanisms of virulence and species differences of leishmania parasites are under the influence of gene expression regulations at posttranscriptional stages. in iran, l. major and l. tropica are known as principal agents of cutaneous leishmaniasis, while l. infantum causes visceral leishmaniasis. methods: as a preliminary study, we compared the proteome mapping of the above three iranian isolates of leishmania species through the 2-dimension electrophoresis (2-de), and identified the prominent proteins by liquid chromatography (lc) mass spectrometry. results : we reproducibly detected about 700 protein spots in each species by using the melanie software. totally, 264 proteins exhibited significant changes among 3 species. forty nine protein spots identified in both l. tropica and l. major were similar in position in the gel, whereas only 35 of l. major proteins and 10 of l. tropica proteins were matched with those of l. infantum. having identified 24 proteins in the three species, we sought to provide possible explanations for their differential expression patterns and discuss their relevance to cell biology. conclusion : the comparison of proteome profiling pattern of the 3 species identified limit up and limit down regulated or absent /present proteins. in addition, the lc-ms data analysis showed that most of the protein spots with differential abundance in the 3 species are involved in cell motility and cytoskeleton, cell signaling and vesicular trafficking, intracellular survival / proteolysis, oxidative stress defense, protein synthesis, protein ubiquitination / proteolysis, and stress related proteins. differentially proteins distributed among the species maybe implicated in host pathogenecity interactions and parasite tropism to cutaneous or visceral tissue macrophages
منابع مشابه
Comparison of the Proteome Profiling of Iranian isolates of Leishmania tropica, L. major and L. infantum by Two-Dimensional Electrophoresis (2-DE) and Mass-spectrometry
BACKGROUND The mechanisms of virulence and species differences of Leishmania parasites are under the influence of gene expression regulations at posttranscriptional stages. In Iran, L. major and L. tropica are known as principal agents of cutaneous leishmaniasis, while L. infantum causes visceral leishmaniasis. METHODS As a preliminary study, we compared the proteome mapping of the above thre...
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چکیده ندارد.
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BACKGROUND In this study, two-dimensional gel electrophoresis (2-DE) method was applied to determine and compare the protein spots expressed in the two field isolates of Leishmania major and recovered from the patients who were clinically sensitive and resistant to Glucantime® treatment. METHODS Leishmania parasites were isolated from the cutaneous lesions of two CL infected patients in Shira...
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عنوان ژورنال:
iranian journal of parasitologyجلد ۱۰، شماره ۴، صفحات ۵۳۰-۵۴۰
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